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Original Article

Online Publishing Date:
06 / 08 / 2024

 


Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh

Mst Meheronnesha, Md. Salauddin, Minakshi Nag, Mir Rowshan Akter, Md. Shofinur Rahaman, Jannatul Ferdous, Md. Fakhruzzaman.


Abstract
This research aimed to characterize Vibrio species from cholera-suspected patients in three hospitals in Dinajpur. We collected 36 stool samples and identified Vibrio spp. using cultural, morphological, and biochemical tests. The highest prevalence was observed in children (18.18%), compared to adolescents (0%), adults (16.67%), and senior adults (16.67%). Cultural identification revealed yellow colonies on TCBS agar, hemolytic colonies on blood agar, pale non-lactose fermenting colonies on MacConkey agar, and white, opaque colonies on nutrient agar. Gram staining showed gram-negative, curved rod-shaped bacteria. Biochemical tests indicated positive reactions for oxidase, catalase, MR, TSI, citrate utilization, MIU, and indole, while VP was negative. Molecular characterization by PCR and gel electrophoresis using 16S rRNA primers confirmed the presence of Vibrio spp. Antibiotic sensitivity testing showed that isolates were sensitive to cotrimoxazole-25µg (21 mm to 30 mm), neomycin-30µg (22 mm), gentamycin-10µg (15 mm), streptomycin-10µg (20 mm), and tetracycline-30µg (17 mm), but resistant to penicillin, mecillinam, and amoxicillin. These findings suggest streptomycin and cotrimoxazole as effective drugs for controlling cholera.

Key words: Vibrio spp., Cholera-suspected patients, Stool samples, Antibiotic resistance, Bangladesh


 
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How to Cite this Article
Pubmed Style

Meheronnesha M, Salauddin M, MN, Akter MR, MSR, Ferdous J, Fakhruzzaman M. Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. Eu J Microbiol Infect Dis. Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040

Web Style

Meheronnesha M, Salauddin M, MN, Akter MR, MSR, Ferdous J, Fakhruzzaman M. Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. https://www.wisdomgale.com/ejmid/?mno=209078 [Access: August 07, 2024]. doi:10.5455/EJMID.20240709044040

AMA (American Medical Association) Style

Meheronnesha M, Salauddin M, MN, Akter MR, MSR, Ferdous J, Fakhruzzaman M. Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. Eu J Microbiol Infect Dis. Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


Vancouver/ICMJE Style

Meheronnesha M, Salauddin M, MN, Akter MR, MSR, Ferdous J, Fakhruzzaman M. Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. Eu J Microbiol Infect Dis, [cited August 07, 2024]; Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


Harvard Style

Meheronnesha, M., Salauddin, . M., , . M. N., Akter, . M. R., , . M. S. R., Ferdous, . J. & Fakhruzzaman, . M. (0) Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. Eu J Microbiol Infect Dis, Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


Turabian Style

Meheronnesha, Mst, Md. Salauddin, Minakshi Nag, Mir Rowshan Akter, Md. Shofinur Rahaman, Jannatul Ferdous, and Md. Fakhruzzaman. 0. Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. European Journal of Microbiology and Infectious Diseases, Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


Chicago Style

Meheronnesha, Mst, Md. Salauddin, Minakshi Nag, Mir Rowshan Akter, Md. Shofinur Rahaman, Jannatul Ferdous, and Md. Fakhruzzaman. "Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh." European Journal of Microbiology and Infectious Diseases Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


MLA (The Modern Language Association) Style

Meheronnesha, Mst, Md. Salauddin, Minakshi Nag, Mir Rowshan Akter, Md. Shofinur Rahaman, Jannatul Ferdous, and Md. Fakhruzzaman. "Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh." European Journal of Microbiology and Infectious Diseases Online First: 06 Aug, 2024. Web. 07 Aug 2024 doi:10.5455/EJMID.20240709044040


APA (American Psychological Association) Style

Meheronnesha, M., Salauddin, . M., , . M. N., Akter, . M. R., , . M. S. R., Ferdous, . J. & Fakhruzzaman, . M. (0) Molecular identification and antibiotic resistance profiling of Vibrio spp. in diarrhoeal patients from Dinajpur, Bangladesh. European Journal of Microbiology and Infectious Diseases, Online First: 06 Aug, 2024. doi:10.5455/EJMID.20240709044040


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