E-ISSN 3041-4156
 
2025, Vol: 2, Issue: 3
2 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Original Article
Online Published: 30 May 2025
 


Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.

Daniel Lotanna Ajaghaku, Uchechukwu Harrison Orji, Paul Chinwuba.


Abstract
Background: This study investigated the combined effects of phenolic and phytoestrogen compounds in Ochna schweinfurthianaon oral glucose tolerance in an ovariectomized rat model of menopause.
Methods: Pulverized root powder of Ochna schweinfurthiana was extracted with methanol, and the extract was partitioned into hexane, ethyl acetate, butanol, and water fractions. The total phenolic and phytoestrogen contents of the extracts and fractions were tested. Ovariectomy was performed via surgical excision of the ovaries along the upper horn. One hour after administration of the combined phytoestrogen- and phenol-rich fractions to the ovariectomized animals, they were administered 2 g/kg D-glucose (p.o). Blood samples were collected at 15, 30, 45, 60, 90, and 120 min after glucose administration to determine the blood glucose concentration. Phytoestrogen: phenol combination groups of five animals received phytoestrogen (PE) 132: Phenol (PH) 251 mg/kg and PE 160: PH 130 mg/kg phytoestrogen and phenol rich fraction combinations, respectively.
Results: The extract contained a considerable quantity of total phenol (142.16 mgGAE/g), which partitioned mostly into the ethyl acetate fraction (phenol-rich fraction), while phytoestrogens were concentrated in the water fraction. The Area under the blood glucose curve (AUC) of the vehicle control group was the highest (27645 mg x min/dl) compared to that of the treatment groups. PE 132:PH 251 mg/kg and PE 160: PH 130 mg/kg recorded 17810 and 16188 mg x min/dl respectively which were lower than the value (18804 mg x min/dl) recorded for the reference drug (estradiol). Conclusion: The combination of phenolic and phytostrogen phytocompounds in Ochna schweinfurthiana improved glucose tolerance, which was impaired by ovariectomy.

Key words: Phenolic compounds, Phytoestrogens, Ovariectomized, Glucose tolerance, Ochna swienfurthiana, Menopause, Insulin sensitivity


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Daniel Lotanna Ajaghaku
Articles by Uchechukwu Harrison Orji
Articles by Paul Chinwuba
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ajaghaku DL, Orji UH, Chinwuba P. Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. J Pharmacol Pharm Res. 2025; 2(3): 87-96. doi:10.5455/JPPR.20250430103757

Web Style

Ajaghaku DL, Orji UH, Chinwuba P. Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. https://www.wisdomgale.com/jppr/?mno=255431 [Access: June 10, 2025]. doi:10.5455/JPPR.20250430103757

AMA (American Medical Association) Style

Ajaghaku DL, Orji UH, Chinwuba P. Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. J Pharmacol Pharm Res. 2025; 2(3): 87-96. doi:10.5455/JPPR.20250430103757


Vancouver/ICMJE Style

Ajaghaku DL, Orji UH, Chinwuba P. Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. J Pharmacol Pharm Res. (2025), [cited June 10, 2025]; 2(3): 87-96. doi:10.5455/JPPR.20250430103757


Harvard Style

Ajaghaku, D. L., Orji, . U. H. & Chinwuba, . P. (2025) Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. J Pharmacol Pharm Res, 2 (3), 87-96. doi:10.5455/JPPR.20250430103757


Turabian Style

Ajaghaku, Daniel Lotanna, Uchechukwu Harrison Orji, and Paul Chinwuba. 2025. Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. Journal of Pharmacological and Pharmaceutical Research, 2 (3), 87-96. doi:10.5455/JPPR.20250430103757


Chicago Style

Ajaghaku, Daniel Lotanna, Uchechukwu Harrison Orji, and Paul Chinwuba. "Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.." Journal of Pharmacological and Pharmaceutical Research 2 (2025), 87-96. doi:10.5455/JPPR.20250430103757


MLA (The Modern Language Association) Style

Ajaghaku, Daniel Lotanna, Uchechukwu Harrison Orji, and Paul Chinwuba. "Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.." Journal of Pharmacological and Pharmaceutical Research 2.3 (2025), 87-96. Print. doi:10.5455/JPPR.20250430103757


APA (American Psychological Association) Style

Ajaghaku, D. L., Orji, . U. H. & Chinwuba, . P. (2025) Evaluation of Combination effects of phenolic and phytoestrogen compounds of Ochna swienfurthiana on oral glucose tolerance in ovariectomized animal model of menopause.. Journal of Pharmacological and Pharmaceutical Research, 2 (3), 87-96. doi:10.5455/JPPR.20250430103757


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About Journal of Pharmacological and Pharmaceutical Research

Journal of Pharmacological and Pharmaceutical Research is an international, peer-reviewed, open access journal, providing a platform for advances in basic ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

Your comments are very important to us

We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below.
 

Emails:

info@wisdomgale.com
editorinchief@ejmanager.com

Office Address:
WisdomGale Publishing
14 Rue de Grand-Bigard,
1082 Brussels
Belgium
 
We will respond within 48 to 72 hours.
 
Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.